UV-Spectroscopy Spectroscopy is an analytical technique based on the interaction of the electromagnetic radiation with matter. Electromagnetic radiation (E.M.R.) – Electromagnetic radiation is a form of energy which is dual in nature. Wave nature - frequency, wave-length and velocity. Particulate nature – individual photon, discrete packet of energy. The energy carried by an E.M.R. or a photon is directly proportional to the frequency, i.e. E = hv =hc/ = hv' c Where h is Planck’s constant h=6.626x10-34J×s Interaction of electromagnetic radiation with Matter The electromagnetic radiation interacts with matter because electrons and molecules in materials are polarizable. Different types of interactions: • Absorption • Reflection • Transmission • Scattering • Refraction Each interaction can disclose certain properties of the matter – When applying E.M.R. of different frequency different type information can be obtained. The energy, E, associated with th...
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